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WCDG meets monthly September to May on the 3rd Wednesday of the month. Meetings are public and include a light dinner & social hour followed by a speaker. To join our mailing list, email Follow us on Twitter
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October Meeting
Wednesday, October 19, 2016
6:00 pm - 8:00 pm at US Pharmacopeia (Directions)

Speaker: Trina Formolo, National Institute of Standards and Technology
Title: Considerations for Optimization of Multi-Attribute Peptide Mapping Protocols

Abstract: Peptide mapping is a widely used methodological platform in the biopharmaceutical industry.  This analytical tool uses the chromatographic profile of enzymatically generated peptides to establish protein identity, evaluate stability and monitor new drug lots for variation.  The use of peptide mapping for multi-attribute method (MAM) applications is becoming more widespread in the industry.  These platforms have been developed to streamline the quality control process by replacing multiple analytical methods with one comprehensive peptide mapping analysis. While a powerful, streamlined data analysis workflow is important in building a successful multi-attribute method (MAM) platform, the platform must also incorporate optimized sample preparation and analysis protocols.  The generation of reliable, meaningful results from any MAM platform begins with quality sample preparation and analysis.  This includes developing robust, reproducible tryptic digestion protocols and optimizing chromatographic separation methods to facilitate the detection and quantitation of critical quality attributes (CQA).

Bio: Dr. Formolo received her BS in biology from the University of Dallas and her PhD in molecular medicine from the Institute for Biomedical Sciences at The George Washington University.   As a research chemist in the Biomolecular Measurement Division within the Material Measurement Laboratory of the National Institute for Standards and Technology (NIST) she focuses on the development of reference materials, methods and data relevant to the biopharmaceutical industry.  Her interests include the mass spectrometry characterization of biotherapeutic proteins.  She recently completed the LC-UV-MS/MS peptide mapping characterization of a recombinant IgG1κ monoclonal antibody (NISTmAb RM 8671) which was recently released as a Reference Material.

Congratulations to Sam Choi, our 2016 winner of the Georges Guiochon travel award for best student poster at the WCDG Open House & Poster Session on September 21!

Sam Choi from George Washington University won the award for his outstanding poster on "CE-nanoESI-MS for Untargeted Proteomic Characterization of Single Embryonic Cells and Small Neuron Populations". He will receive complementary travel to a separations-related conference of his choice. Congratulations, Sam!

We also want to acknowledge our Honorable Mentions, Erika Portero and Rosemary Onjiko from George Washington University, and thank all of our poster presenters, vendors, and attendees for making this year's event a success! Download 2016 Program



Past Events


2016 Open House & Poster Session

September 22, 2016 at Bethesda North Marriott Conference Center


3rd Annual Chromatography & Separations Community Mixer

March 10, 2015 at Generations Hall New Orleans


2014 Open House & Poster Session

Rockville, MD


2014 Chromatography Community Mixer

Chicago, IL